Cells simultaneously assemble and maintain different actin cytoskeleton networks of distinct organizations and dynamics for fundamental processes such as polarization, endocytosis, motility and division. How does a shared pool of actin monomer building blocks self-organize into distinct actin filament networks of specific densities, sizes and function within a common cellular cytoplasm? Our research group is investigating the molecular mechanisms that govern how diverse actin cytoskeleton networks self-organize through (1) competition for a limited supply of actin monomers, (2) network-specific sorting of actin binding proteins, and (3) the effects of different combinations of actin binding proteins on actin filament network organization, dynamics and function. We take advantage of complementary experiments in live cells and with purified components. These interdisciplinary techniques include genetics, time-lapse fluorescence microscopy of cells, biochemistry, biophysics, in vitro reconstitution and multi-color single molecule TIRF microscopy imaging.
Yale University
New Haven, CT
Postdoctoral Fellow - Biochemistry and Biophysics
2005
Purdue University
West Lafayette, IN
PhD - Biological Sciences
2001
Ohio Wesleyan University
Delaware, OH
BA - Botany and Microbiology
1995
Cracked actin filaments as mechanosensitive receptors.
Cracked actin filaments as mechanosensitive receptors. Biophys J. 2024 Oct 01; 123(19):3283-3294.
PMID: 38894540
A step-by-step guide to fragmenting bundled actin filaments.
A step-by-step guide to fragmenting bundled actin filaments. J Cell Biol. 2024 06 03; 223(6).
PMID: 38748453
Prediction of the essential intermolecular contacts for side-binding of VASP on F-actin.
Prediction of the essential intermolecular contacts for side-binding of VASP on F-actin. Cytoskeleton (Hoboken). 2024 Aug; 81(8):382-392.
PMID: 38647032
Arp2/3 complex- and formin-mediated actin cytoskeleton networks facilitate actin binding protein sorting in fission yeast.
Arp2/3 complex- and formin-mediated actin cytoskeleton networks facilitate actin binding protein sorting in fission yeast. Eur J Cell Biol. 2024 Jun; 103(2):151404.
PMID: 38493594
Highly flexible PEG-LifeAct constructs act as tunable biomimetic actin crosslinkers.
Highly flexible PEG-LifeAct constructs act as tunable biomimetic actin crosslinkers. Soft Matter. 2024 Jan 31; 20(5):971-977.
PMID: 38190228
Multi-monoubiquitylation controls VASP-mediated actin dynamics.
Multi-monoubiquitylation controls VASP-mediated actin dynamics. J Cell Sci. 2024 01 15; 137(2).
PMID: 38277158
Multi-monoubiquitination controls VASP-mediated actin dynamics.
Multi-monoubiquitination controls VASP-mediated actin dynamics. J Cell Sci. 2024 Jan 12.
PMID: 38214186
Reconstitution of the transition from a lamellipodia- to filopodia-like actin network with purified proteins.
Reconstitution of the transition from a lamellipodia- to filopodia-like actin network with purified proteins. Eur J Cell Biol. 2023 Dec; 102(4):151367.
PMID: 37890285
Multi-monoubiquitination controls VASP-mediated actin dynamics.
Multi-monoubiquitination controls VASP-mediated actin dynamics. bioRxiv. 2023 Jul 17.
PMID: 37503134
Cracked actin filaments as mechanosensitive receptors.
Cracked actin filaments as mechanosensitive receptors. bioRxiv. 2023 Jun 28.
PMID: 37425801
BSD Distinguished Educator and Mentor Award
University of Chicago
2011